Comparative Transcriptome Analysis Identifies Potentially Relevant Genes in Rubber Clones with a High Latex Yield (Hevea brasiliensis)

Since the 1950s, rubber tree (Hevea brasiliensis) has significantly contributed to Malaysia's agricultural economy due its capability produce high-value natural rubber. Due presence of cis-1,4-polyisoprene, exhibits unique properties such as flexibility, high elasticity, and efficient heat dispersion. Cis-1,4-polyisoprene is synthesized via two distinct metabolic pathways: isoprenoid biosynthesis. RNA sequencing was performed on 12 specimens from bark, leaf, latex tissues clones, RRIM 3001 (high yield) 712 (low yield). After quality assessment, these generated a mean 73,816,244 clean reads. The differential expression analysis revealed that Acetyl-CoA-Acetyltransferase (AACT) Diphosphomevalonate Decarboxylase (MVD) genes (via MVA pathway), well Small Rubber Particle Protein (SRPP) Elongation Factor (REF) biosynthesis were overexpressed in compared 712. transcription factor Ethylene Response (ERF) also found be more highly expressed than Five clones with random yield characteristics used quantitative amplification validate AACT, MVD, SRPP, REF, ERF genes. shows five consistently clones.